70% Liposome NMN

70% Liposome NMN is a powdered raw material made by encapsulating β-nicotinamide mononucleotide (NMN, CAS 1094-61-7) using nanoliposome technology. The product typically appears as a white to pale yellow powder, with 70% NMN as the core component and the remainder primarily consisting of phospholipids (such as non-GMO sunflower phospholipids or soybean phospholipids) used as a carrier. This technology aims to protect NMN from digestive breakdown through the liposome structure, thereby enhancing its stability and bioavailability. It is a common functional ingredient in dietary supplements and anti-aging compound products.

1. NMN Content Testing
This test confirms whether the actual 70% Liposome NMN content in the product meets the 70% standard claimed on the label.

• Standard Requirement: 70% ± 5% (i.e., 65%-75%)
• Test Method: HPLC (High Performance Liquid Chromatography)
• Method Description: After establishing a standard curve and verifying the linearity, precision, and accuracy of the method, the sample is quantitatively measured. In a real-world example, our COA showed an actual NMN content of 70.7% for 70% Liposome NMN, meeting the standard requirement.
• Test Purpose: To ensure that the true NMN content in the product matches the label, preventing false labeling or insufficient content.

2.Physicochemical Properties

3.Heavy Metal Testing

4.Microbiological Testing

5. Stereoisomeromer Identification
NMN exists in two stereoisomers, α and β, of which only β-NMN has biological activity. α-NMN cannot be recognized by NAD+ synthase and does not possess anti-aging effects.

• Standard Requirements: β-NMN purity ≥98% (isomer level)
• Detection Method: LC-MS/MS (Liquid Chromatography-Tandem Mass Spectrometry)

Detection Purpose: To distinguish between the active ingredient (β-type) and the ineffective ingredient (α-type). A 2025 study analyzed eight commercially available NMN supplements and found significant differences in isomer composition among different products, with some products containing undeclared or inaccurately labeled ingredients. Therefore, isomer identification is a crucial and indispensable step in quality control.

6.Liposome structure and encapsulation efficiency

Method Description: Encapsulation efficiency is typically determined using ultracentrifugation or size exclusion chromatography to separate free NMN from NMN encapsulated in liposomes, and then the content is measured separately. Studies have shown that HPLC has good resolution, simplicity, and specificity for determining NMN liposome encapsulation efficiency.

Structural Confirmation: Transmission electron microscopy (TEM) allows direct observation of liposome morphology to confirm the presence of a complete "dark-light-dark" three-layer membrane structure.

Objective of the Test: To ensure that NMN is effectively encapsulated in liposomes to achieve the designed goals of improving stability and bioavailability. If the encapsulation efficiency is too low (e.g., below 20%), the liposome technology loses its practical significance.

I. Significantly Enhanced Stability
The liposome structure provides physical protection for NMN:

High acid resistance: After treatment in simulated gastric juice (pH=2.0) for 4 hours, the retention rate still exceeds 80%.

Good heat resistance: The phospholipid layer effectively protects NMN from degradation under high temperatures.

Storage stability: The lyophilized powder form facilitates transportation and long-term storage.

2. Highly Effective Increase in NAD+ Levels
Clinical studies have confirmed its effectiveness:

Significant increase: A 4-week double-blind trial showed that after daily administration of liposomal NMN, blood NAD+ levels increased from 28.6 µM to 52.5 µM. µM, an increase of 84%

Long-lasting effect: 4 weeks after stopping supplementation, NAD+ levels remained significantly higher than baseline.

3. Sustained-release properties support long-lasting effects. The sustained-release effect brought by liposome technology:

Stable release: NMN is slowly released from liposomes, avoiding rapid rises and falls in plasma concentration.

All-day support: The duration of action can last for 8-12 hours, providing a sustained substrate supply for NAD+-dependent enzymes such as Sirtuins.

4. Improved cellular uptake efficiency. The structure of liposomes is highly similar to that of cell membranes:

Fusion absorption: Liposomes can directly fuse with cell membranes, delivering NMN into the cell interior.

Lymphatic absorption pathway: Absorption can be achieved through the lymphatic system, bypassing the first-pass metabolism in the liver.